Blood Collection by Capillary Puncture Method

Objective

Students shall be able to collect blood by capillary puncture

Capillary Puncture
Capillary Puncture

Principle

Capillary puncture is used to collect blood for
various tests when a small amount of blood is required.

Requirements

  1. Disposable needles or lancet,
  1. spirit swab
  2. Other as per test e.g. Sahli’s pipette in case of
    Hb estimation or slides in case of blood groups.
  1. Sites of blood collection
     Ball of Finger
     Ear lobe
      heel in case of infants

Procedure

1. First clean the site with 95% alcohol or spirit and allow it to dry completely.

2. Hold the ball of a finger tightly with the thumb and index finger.

3. With the help of a disposable needle or lancet
give a bold prick.  It should be deep 2-4 mm

4. Squeezing tends to stop the flow of blood, also adding tissue fluid to
the drop of blood, which gives inaccurate
count, so squeezing should not be done.

5. Wipe off the first drop of blood and next is
used for testing.

6. After collection place a cotton swab over the
punctured site. Apply slight pressure to stop
blood flow.

Observations

The procedure used to collect blood by capillary puncture is described in the form of images as follows

finger puncture Method

Result

Blood is collected and labeled as per requirement.

Also Read

 

  1. Bile Salt (Hays Sulphur Method)
  2. Stool Examination: What You Need to Know

  3. Estimation of occult blood in stool by Benzidine method.
  4. Unlocking the Secrets of Urine: A Comprehensive Guide to Urine Examination

  5. Glucosuria (Benedict Method)
  6. Ketone Body (Rotheras, Gerhardt’s and Strip Method)
  7. Proteinuria (Heat and acetic acid, Sulphur Salicylic Acid, Hellers or Nitric acid, and Esbach albuminometer Method)
  8. Bence Jones Proteinuria (HCl Method)
  9. Urobilinogen (Ehrlich Method)
  10. Bile Pigment (Fouchets Method)
  11. Occult Blood (Orthotoluidine and Benzidine Method).
  12. Sedimentation Preparation
  13. Slide Preparation
  14. Microscopic Examination.
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Sandeep_Sir_Medico

Chairman/Founder
Samrat Group
Ex. LT SRH Hospital NCVT Delhi Govt.
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Created by Sandeep Sir Medico

"Mastering Urine Examination: 20 MCQs with Answers for Competitive and Academic Success"

1 / 20

1. The presence of which type of crystals in urine is associated with cystinuria?

2 / 20

2. Oval fat bodies in urine are often seen in patients with:

3 / 20

3. When is the presence of renal tubular cells in urine most concerning?

4 / 20

4. What is the primary function of transitional epithelial cells?

5 / 20

5. What is the primary function of casts in urine?

6 / 20

6. What is the primary function of casts in urine?

7 / 20

7. Which type of crystal is often found in acidic urine and can be associated with gout?

8 / 20

8. In a clean-catch urine sample, what is the primary source of squamous epithelial cells?

9 / 20

9. Dysmorphic red blood cells in urine are suggestive of:

10 / 20

10. Which of the following is not a type of white blood cell?

11 / 20

11. What is the most common type of epithelial cell found in urine?

12 / 20

12. The presence of eosinophilic casts in urine may be indicative of:

13 / 20

13. A cast composed of lipid material is called a:

14 / 20

14. Which type of crystal is often associated with ammonium urate?

15 / 20

15. Which type of crystal is often associated with a strong ammonia odor in urine?

16 / 20

16. Which type of crystal may appear as "sheaves of wheat" in urine?

17 / 20

17. Which type of cell is a hallmark of chronic renal disease?

18 / 20

18. Hyaline casts are primarily composed of:

19 / 20

19. The presence of urate crystals in urine may appear as:

20 / 20

20. The presence of white blood cell casts in urine may indicate:

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