“Assessing Ketonuria Using the Rothera’s Method: A Comprehensive Guide”

:Aim:

“Assessing Ketonuria Using the Rothera’s Method: A Comprehensive Guide”

 

Principle:-

Ketone body combines with alkaline sodium nitro-prusside and form purple color complex.

Requirement:-

Urine, test tube, tube rack, centrifuge, sodium nitro prusside, ammonium sulphate, liqual ammonia dropper etc.

Procedure:-

Take 5 ml urine in a test tube saturated urine sample with ammonium sulphate and add few crystal of sodium nitro prusside and mix well, add few drop of liqual ammonia with the help of test tube wall and wait for 5 minute.

Interpretation:-

Appearance of purple color ring at the junction, it indicates presence of Ketone body in urine.

Result:-

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Clinical Significance:-

Cause of Ketonuria due to diabetes mellitus, dehydration and high grade fever.

Also Read:

 

  1. Bile Salt (Hay’s Sulphure Method)

  2. “Unlocking the Secrets of Urine: A Comprehensive Guide to Urine Examination”

  3. Glucoseuria (Benedict Method)
  4. Ketone Body (Rotheras, Gerhardt’s and Strip Method)
  5. Proteinuria (Heat and acetic acid, Sulpho Salicylic Acid,Hellers or Nitric acid, and Esbach albuminometer Method)
  6. Bence Jones Proteinuria (HCl Method)
  7. Urobilinogen (Ehrlich Method)
  8. Bile Pigment (Fouchets Method)
  9. Occult Blood (Orthotoluidine and Benzidine Method).
  10. Sedimentation Preparation
  11. Slide Preparation
  12. Microscopic Examination.
Reference books
  1. “Clinical Chemistry: Techniques, Principles, Correlations” by Michael L. Bishop and Edward P. Fody: This comprehensive text covers various aspects of clinical chemistry, including urinalysis. It’s widely used in medical and clinical laboratory education.
  2. “Henry’s Clinical Diagnosis and Management by Laboratory Methods” by Richard A. McPherson and Matthew R. Pincus: This is a well-regarded resource that provides insights into laboratory tests, including urinalysis, and their clinical applications.
  3. “Tietz Textbook of Clinical Chemistry and Molecular Diagnostics” by Nader Rifai, Andrea R. Horvath, and Carl T. Wittwer: This textbook offers a broad view of clinical chemistry, including urinalysis and molecular diagnostics, making it a valuable resource for students and professionals.
  4. “Urinalysis and Body Fluids” by Susan King Strasinger and Marjorie Schaub Di Lorenzo: This book is specifically focused on urinalysis and the examination of other body fluids. It is used in many medical laboratory technician programs.
  5. “Urinalysis in Clinical Laboratory Practice” by John A. Lott and W. Gregory Miller: This book provides a detailed look at the theory and practice of urinalysis, making it suitable for those studying or working in clinical laboratories.
  6. “Urine Analysis: A Color Atlas and Text” by David S. Goldfarb and Alan S. L. Yu: This text combines visual representations with explanatory text to aid in the understanding and interpretation of urine analysis.
  7. “Pathology Practical Book” by Harsh Mohan: Third Edition.
  8. Internet, Wikipedia
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Sandeep_Sir_Medico

Chairman/Founder
Samrat Group
Ex. LT SRH Hospital NCVT Delhi Govt.
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Created by Sandeep Sir Medico

"Mastering Urine Examination: 20 MCQs with Answers for Competitive and Academic Success"

1 / 20

1. When is the presence of renal tubular cells in urine most concerning?

2 / 20

2. Which type of cell is a hallmark of chronic renal disease?

3 / 20

3. In a clean-catch urine sample, what is the primary source of squamous epithelial cells?

4 / 20

4. A cast composed of lipid material is called a:

5 / 20

5. The presence of which type of crystals in urine is associated with cystinuria?

6 / 20

6. What is the most common type of epithelial cell found in urine?

7 / 20

7. Hyaline casts are primarily composed of:

8 / 20

8. The presence of urate crystals in urine may appear as:

9 / 20

9. Which type of crystal may appear as "sheaves of wheat" in urine?

10 / 20

10. The presence of white blood cell casts in urine may indicate:

11 / 20

11. What is the primary function of casts in urine?

12 / 20

12. The presence of eosinophilic casts in urine may be indicative of:

13 / 20

13. Oval fat bodies in urine are often seen in patients with:

14 / 20

14. What is the primary function of transitional epithelial cells?

15 / 20

15. Which of the following is not a type of white blood cell?

16 / 20

16. Which type of crystal is often found in acidic urine and can be associated with gout?

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17. Dysmorphic red blood cells in urine are suggestive of:

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18. Which type of crystal is often associated with ammonium urate?

19 / 20

19. What is the primary function of casts in urine?

20 / 20

20. Which type of crystal is often associated with a strong ammonia odor in urine?

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