Medical

Estimating Hemoglobin Levels using Drabkin’s or Cyanmethemoglobin Method

Introduction Estimating hemoglobin levels using the Drabkin’s or cyanmethemoglobin method is a common laboratory procedure. Here is a practical protocol for estimating hemoglobin using the cyanmethemoglobin method: Aim: To estimate Hemoglobin by Drabkin’s or cyanmethemoglobin method. Principle: When blood is mixed with Drabkin’s reagent containing K cyanide & K ferricyanide hemoglobin reacts with ferricyanide to

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haemoglobin

Estimation of Haemoglobin by Sahli’s or Acid Haematin Method

Aim: Estimation of blood haemoglobin by Sahli’s or acid haematin method Summary: Haemoglobin is a conjugated protein synthesized inside the immature erythrocyte in the red bone marrow it consists of two components 1. Heme ( iron + protoporphyrin ) and 2. globin (amino acid chain). It gives a red color to blood. Principle: When blood

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RBC Count Step-by-Step Guide: How to Perform an Accurate RBC Count Using Neubauer’s Chamber

Aim: To perform RBC count by using Neubauer’s chamber. Principle: For RBC Count the blood specimen is diluted 1:200 with the R.B.C. diluting fluid & cells are counted under high power by using a counting chamber. The number of cells in undiluted blood are calculated & reported as the number of RBC/cumm of whole blood.

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Tissue-processing

Chapter-1 Histotechniques: Tissue Processing Step by Step

  TISSUE PROCESSING When studying tissues under a microscope, proper tissue processing is crucial to obtain accurate and high-quality results. Histotechniques, also known as histology techniques, involve following series of steps Fixation, Dehydration, Clearing, Infiltration, Embedding, Trimming, Section cutting, staining, mounting and labeling etc. to prepare tissues for microscopic examination. FIXATION AND FIXATIVES   Primary aim:

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DMLT Question Paper 2019: Paper I – General Laboratory Principles, Equipment & HAP

DMLT Question Paper 2019: Paper I The DMLT (Diploma in Medical Laboratory Technology) is a course that prepares individuals to work as laboratory technicians in medical settings. As part of their training, students are required to pass various theoretical and practical exams. One such exam is the DMLT Question 2019 Paper I, which focuses on

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Proteinuria

Proteinuria Test by Heat Acetic Acid Method

Proteinuria is a condition characterized by the presence of excess protein in the urine. It is an important indicator of kidney function and can be a sign of various underlying health issues. To diagnose proteinuria, healthcare professionals often use the heat acetic acid method. The heat acetic acid method is a simple and reliable test

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