Estimation of Urobilinogen Test by Ehrlich Method

The estimation of urobilinogen is an important diagnostic test used to assess liver function and detect certain medical conditions. One of the commonly used methods for this estimation is the Ehrlich method. In this blog post, we will discuss the procedure and significance of the Ehrlich method for urobilinogen test.

Aim:-

Estimation of Urobilinogen test by Ehrlich method.

Principle:-

Urobilinogen react with P – dimethyl amino benzaldehyde in
acidic medium (in Ehrlich reagent) to form pink color compound.

Requirement:-

Centrifuge, test tube, pipette, 10 g/dl Barium chloride,
Ehrlich reagent.

Procedure:-

1. Place 3 to 4 ml urine in a centrifuge tube by using a pipette.
2. Add equal amount of 10 g/dl Barium chloride and mix well.
3. Centrifuge at 1500 RPM for 10 minutes and place supernent another tube.
4. Add about 0.5 ml of Ehrlich reagent to supernent.

Observation:-

Development of pale pink color Urobilinogen present normal while development of cherry red color Urobilinogen presents high.

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"Mastering Urine Examination: 20 MCQs with Answers for Competitive and Academic Success"

1 / 20

1. In a clean-catch urine sample, what is the primary source of squamous epithelial cells?

2 / 20

2. The presence of white blood cell casts in urine may indicate:

3 / 20

3. Which type of crystal may appear as "sheaves of wheat" in urine?

4 / 20

4. What is the primary function of casts in urine?

5 / 20

5. Which of the following is not a type of white blood cell?

6 / 20

6. What is the primary function of casts in urine?

7 / 20

7. Which type of crystal is often associated with a strong ammonia odor in urine?

8 / 20

8. Which type of crystal is often associated with ammonium urate?

9 / 20

9. Which type of cell is a hallmark of chronic renal disease?

10 / 20

10. Hyaline casts are primarily composed of:

11 / 20

11. When is the presence of renal tubular cells in urine most concerning?

12 / 20

12. Dysmorphic red blood cells in urine are suggestive of:

13 / 20

13. The presence of eosinophilic casts in urine may be indicative of:

14 / 20

14. The presence of urate crystals in urine may appear as:

15 / 20

15. What is the primary function of transitional epithelial cells?

16 / 20

16. What is the most common type of epithelial cell found in urine?

17 / 20

17. Oval fat bodies in urine are often seen in patients with:

18 / 20

18. The presence of which type of crystals in urine is associated with cystinuria?

19 / 20

19. Which type of crystal is often found in acidic urine and can be associated with gout?

20 / 20

20. A cast composed of lipid material is called a:

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