Microscopic Examination of the Semen

“Introduction”

Microscopic examination of the semen is an important diagnostic tool used in the field of reproductive medicine. It involves the analysis of various parameters of the semen sample to assess the fertility potential of an individual. This procedure is commonly performed as part of a comprehensive fertility evaluation.

“MICROSCOPIC EXAMINATION OF THE SEMEN”

1. Sperm Counting:-

Sperm counting is done by Neubauer’s chamber by diluting of semen in 1:20 with the help of WBC pipette or other and count spermatozoa in two TLC counting chamber

Requirement:-

Neubauer’s chamber, WBC pipette, semen, diluting fluid, cover slip, microscope etc.

Procedure:-

1.Dilute the semen in 1:20 with the help of WBC pipette.
2.Place a cover slip on Neubauer’s chamber change it aseptically.
3.Wait for two minute allow to stepdown spermatozoa in chamber.
4.Place the chamber under microscope and count spermatozoa in two TLC corner
squire and calculate the number of spermatozoa

Calculation:-

Result:-

Normal Value:-

Normally = > 60 million/ ml
Abnormally = <20 million / ml.

2. Examination Of Motility:-

Motility examine of semen done by Microscopic Examination of the Semen by preparation of wet mount preparation of semen slide by placing a drop of liquefied semen on a glass slide and cover it with cover slip.
Normally within two hours of ejaculation more than 60% spermatozoa are vigorously motile in normal semen.

3. Morphology Examination Of Semen:–

Prepare a thin smear from liquefied semen on a glass slide stain it with any of the
Romanowski stain, PAP, or H&E stain. Observe at list 200 spermatozoa for any abnormalities in there morphology. Normally 80% spermatozoa normal in morphology.

The abnormal form of spermatozoa are with double head swollen head and pointed head double tail and other abnormal form of spermatozoa are seen

Also Read:

1. Physical or gross Examination Of Semen
2. Chemical Examination of semen

3. Stool Examination: What You Need to Know

4. Estimation of occult blood in stool by Benzidine method.
5. Microscopic examination of stool
6. Bile Salt (Hay^’s Sulphur Method)

7. “Unlocking the Secrets of Urine: A Comprehensive Guide to Urine Examination”

8. Glucoseuria (Benedict Method)
9. Ketone Body (Rotheras, Gerhardt’s and Strip Method)
10. Proteinuria (Heat and acetic acid, Sulpho Salicylic Acid,Hellers or Nitric acid, and Esbach albuminometer Method)
11. Bence Jones Proteinuria (HCl Method)
12. Urobilinogen (Ehrlich Method)
13. Bile Pigment (Fouchets Method)
14. Occult Blood (Orthotoluidine and Benzidine Method).
15. Sedimentation Preparation
16. Slide Preparation
17. Microscopic Examination.

Reference books

1. “Clinical Chemistry: Techniques, Principles, Correlations” by Michael L. Bishop and Edward P. Fody: This comprehensive text covers various aspects of clinical chemistry, including urinalysis. It’s widely used in medical and clinical laboratory education.
2. “Henry’s Clinical Diagnosis and Management by Laboratory Methods” by Richard A. McPherson and Matthew R. Pincus: This is a well-regarded resource that provides insights into laboratory tests, including urinalysis, and their clinical applications.
3. “Tietz Textbook of Clinical Chemistry and Molecular Diagnostics” by Nader Rifai, Andrea R. Horvath, and Carl T. Wittwer: This textbook offers a broad view of clinical chemistry, including urinalysis and molecular diagnostics, making it a valuable resource for students and professionals.
4. “Urinalysis and Body Fluids” by Susan King Strasinger and Marjorie Schaub Di Lorenzo: This book is specifically focused on urinalysis and the examination of other body fluids. It is used in many medical laboratory technician programs.
5. “Urinalysis in Clinical Laboratory Practice” by John A. Lott and W. Gregory Miller: This book provides a detailed look at the theory and practice of urinalysis, making it suitable for those studying or working in clinical laboratories.
6. “Urine Analysis: A Color Atlas and Text” by David S. Goldfarb and Alan S. L. Yu: This text combines visual representations with explanatory text to aid in the understanding and interpretation of urine analysis.
7. “Pathology Practical Book” by Harsh Mohan: Third Edition.
8. Internet, Wikipedia