Occult Blood Test in Urine by Orthotoluidine Method

The occult blood test in urine is a diagnostic procedure used to detect the presence of blood in the urine that is not visible to the naked eye. One of the methods used for this test is the Orthotoluidine method. In this blog post, we will discuss how to perform the occult blood test in urine using the Orthotoluidine method.

Aim:-

Perform occult blood test in urine by Orthotoluidine method.

Requirement

Test tube, Orthotoluidine, glacial acetic acid, H2O2, urine pipette etc.

Procedure:-

1. Take 2 ml urine in a test tube.
2. Add a solution of 1 ml of Orthotoluidine in glacial acetic acid.
3. Add few drop of H2O2.

Interpretation:

Blue or green color indicates presence of blood in urine.

Occult Blood Test in Urine

Also Read

 

  1. Bile Salt (Hay’s Sulphur Method)

  2. Stool Examination: What You Need to Know

  3. Estimation of occult blood in stool by Benzidine method.

  4. “Unlocking the Secrets of Urine: A Comprehensive Guide to Urine Examination”

  5. Glucosuria (Benedict Method)
  6. Ketone Body (Rotheras, Gerhardt’s and Strip Method)
  7. Proteinuria (Heat and acetic acid, Sulphur Salicylic Acid, Hellers or Nitric acid, and Esbach albuminometer Method)
  8. Bence Jones Proteinuria (HCl Method)
  9. Urobilinogen (Ehrlich Method)
  10. Bile Pigment (Fouchets Method)
  11. Occult Blood (Orthotoluidine and Benzidine Method).
  12. Sedimentation Preparation
  13. Slide Preparation
  14. Microscopic Examination.
Reference books
  1. “Clinical Chemistry: Techniques, Principles, Correlations” by Michael L. Bishop and Edward P. Fody: This comprehensive text covers various aspects of clinical chemistry, including urinalysis. It’s widely used in medical and clinical laboratory education.
  2. “Henry’s Clinical Diagnosis and Management by Laboratory Methods” by Richard A. McPherson and Matthew R. Pincus: This is a well-regarded resource that provides insights into laboratory tests, including urinalysis, and their clinical applications.
  3. “Tietz Textbook of Clinical Chemistry and Molecular Diagnostics” by Nader Rifai, Andrea R. Horvath, and Carl T. Wittwer: This textbook offers a broad view of clinical chemistry, including urinalysis and molecular diagnostics, making it a valuable resource for students and professionals.
  4. “Urinalysis and Body Fluids” by Susan King Strasinger and Marjorie Schaub Di Lorenzo: This book is specifically focused on urinalysis and the examination of other body fluids. It is used in many medical laboratory technician programs.
  5. “Urinalysis in Clinical Laboratory Practice” by John A. Lott and W. Gregory Miller: This book provides a detailed look at the theory and practice of urinalysis, making it suitable for those studying or working in clinical laboratories.
  6. “Urine Analysis: A Color Atlas and Text” by David S. Goldfarb and Alan S. L. Yu: This text combines visual representations with explanatory text to aid in the understanding and interpretation of urine analysis.
  7. “Pathology Practical Book” by Harsh Mohan: Third Edition.
  8. Internet, Wikipedia
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Samrat Group
Ex. LT SRH Hospital NCVT Delhi Govt.
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Created by Sandeep Sir Medico

"Mastering Urine Examination: 20 MCQs with Answers for Competitive and Academic Success"

1 / 20

1. Hyaline casts are primarily composed of:

2 / 20

2. Dysmorphic red blood cells in urine are suggestive of:

3 / 20

3. What is the primary function of casts in urine?

4 / 20

4. The presence of which type of crystals in urine is associated with cystinuria?

5 / 20

5. Which type of crystal is often associated with ammonium urate?

6 / 20

6. Which type of crystal is often associated with a strong ammonia odor in urine?

7 / 20

7. Which type of crystal may appear as "sheaves of wheat" in urine?

8 / 20

8. The presence of urate crystals in urine may appear as:

9 / 20

9. Which of the following is not a type of white blood cell?

10 / 20

10. Oval fat bodies in urine are often seen in patients with:

11 / 20

11. The presence of eosinophilic casts in urine may be indicative of:

12 / 20

12. What is the primary function of casts in urine?

13 / 20

13. Which type of cell is a hallmark of chronic renal disease?

14 / 20

14. What is the most common type of epithelial cell found in urine?

15 / 20

15. In a clean-catch urine sample, what is the primary source of squamous epithelial cells?

16 / 20

16. Which type of crystal is often found in acidic urine and can be associated with gout?

17 / 20

17. A cast composed of lipid material is called a:

18 / 20

18. What is the primary function of transitional epithelial cells?

19 / 20

19. The presence of white blood cell casts in urine may indicate:

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20. When is the presence of renal tubular cells in urine most concerning?

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